Optimising testing for phospholipid antibodies

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Optimising testing for phospholipid antibodies.

AIM To compare anticardiolipin (ACL) and anti-beta2 glycoprotein 1 (beta2gp1) enzyme linked immunosorbent assays (ELISAs) in the diagnosis of antiphospholipid syndrome (APS) and to incorporate these results into a meta-analysis of published data. METHOD Three representative commercial ACL ELISAs and an in house beta2gp1 assay were optimised and then assessed on 124 sera from normal donors, pa...

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Anti-phospholipid/Cofactor Antibodies

Following a meeting at Sapporo, Japan, an international consensus statement on preliminary classification criteria for definite anti-phospholipid syndrome was published.1 This set of criteria is divided into the clinical part which includes vascular thrombosis and pregnancy morbidity and the laboratory part which includes anti-cardiolipin (aCL) antibody and lupus anti-coagulant tests. Diagnosis...

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Sulfatides: targets for anti-phospholipid antibodies.

BACKGROUND Sulfatides are sulfated glycosphingolipids expressed on the surface of erythrocytes, leukocytes, and platelets. Sulfatides interact with several cell adhesion molecules involved in hemostasis. Beta2-glycoprotein I is an anionic phospholipid-binding plasma protein, and the phospholipid-bound form is the target for most anti-phospholipid antibodies that are associated with recurrent th...

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Anti-phospholipid antibodies and pregnancy

The presence of antiphospholipid antibodies is linked to an increased rate of repeated spontaneous abortions and fetal deaths, that in fact belong to the clinical spectrum of the antiphospholipid syndrome. The pathogenic role of antiphospholipid antibodies was clearly shown in experimental animals that, when infused during pregnancy, develop placental insufficiency and miscarriages. In addition...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 2001

ISSN: 0021-9746

DOI: 10.1136/jcp.54.9.693